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Versha Parcha , Manoj Gahlot , M. S. M. Rawat

Abstract

Oils and fats are integral part of human diet and to match the demands of ever increasing population, there is a continuous search for new edible oils. In the present study, seed coats of Dysoxylum binectariferum were examined. The plant produces seeds in abundant quantity and the seed coats are found to contain fine oil in 40% yield. The results of gas liquid chromatographic analysis indicate that the oil is rich in unsaturated fatty acids like oleic, linoleic acids which are the essential requirement of human diet. The oil also contains palmitoleic, eicosenoic and linolenic acids and saturated fatty acids i.e. myristic, stearic, arachidic and palmitic acids and is proven to be non-toxic on animal experimentation's (rats). Since the oil is present in good yield, is non-toxic and contains essential fatty acids so has to promise to be used as an alternate source of edible oil.

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Ravi Prakash Mahor ¹, Versha Parcha ², Yogendra Singh ¹, Rajiv Sharma ³, Anil Bhandari ¹

Affiliations
1 Jodhpur National University, Jodhpur, Rajasthan, IN
2 Department of Pharmaceutical Sciences, SBS PG Institute of Biomedical Sciences and Research, Balawala, Dehradun – 248 161, Uttarakhand, IN
3 Alembic Ltd., Baddi, Himachal Pradesh, IN

Abstract

A simple, precise and accurate HPLC method has been validated to measure lamivudine and zidovudine at single wavelength (270 nm) in order to assess in vitro drug release profile of drug from tablet formulation. The chromatographic separation was achieved on a Hypersil SS C18 (250 mm × 4.6 mm, 5μm) analytical column. Methanol was used as the mobile phase, at a flow rate of 1 ml/min. The release of lamivudine and zidovudine from in dissolution medium (distilled water) and in the reference dissolution medium (0.1 N HCl) was studied using the USP type II apparatus. The parameters similarity factor, according to current FDA guidelines, dissolution efficiency, t50 and dissolution rate constant (k) were employed to compare dissolution profiles. It was concluded that test dissolution method was equivalent to the reference dissolution method.

Keywords

Lamivudine, Zidovudine, HPLC, Validation, Dissolution Efficiency, Similarity Factor, Dissolution Rate Constant.

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